Monitoring the enzymatic polymerization of 4-phenylphenol by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: a novel approach.
Identifieur interne : 005400 ( Main/Exploration ); précédent : 005399; suivant : 005401Monitoring the enzymatic polymerization of 4-phenylphenol by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: a novel approach.
Auteurs : Peng Xu [États-Unis] ; Jayant Kumar ; Lynne Samuelson ; Ashok L. CholliSource :
- Biomacromolecules [ 1525-7797 ]
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Dérivés du biphényle, Horseradish peroxidase.
- synthèse chimique : Polymères.
- Catalyse, Peroxyde d'hydrogène, Spectrométrie de masse MALDI.
English descriptors
- KwdEn :
- MESH :
- chemical , chemical synthesis : Polymers.
- chemical , metabolism : Biphenyl Compounds, Horseradish Peroxidase.
- methods : Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
- Catalysis, Hydrogen Peroxide.
Abstract
Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry is a powerful tool for polymer characterization. It has been used to understand the enzymatic polymerization of 4-phenylphenol and to monitor number average molecular weight and weight average molecular weight of the polymer as a function of systematic addition of hydrogen peroxide (H(2)O(2)) in the reaction. A novel method, an introduction of internal standard for quantification of data, has been developed for MALDI-TOF MS to investigate the fate of each mers during the reaction. The preliminary data suggest that this approach provides new insight on the enzymatic synthesis, which is not available by other techniques. For the first time, we are able to understand the fate of several mers as a function of reaction conditions. The relative content of each mer increases with the addition of H(2)O(2), except for dimer and trimer. For example, the concentration of dimer species decreases as a function of H(2)O(2). On the other hand, the concentration of trimer species increases first and then decreases in the course of the reaction.
DOI: 10.1021/bm0255600
PubMed: 12217031
Affiliations:
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Le document en format XML
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Cholli</name></author></analytic><series><title level="j">Biomacromolecules</title><idno type="ISSN">1525-7797</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biphenyl Compounds (metabolism)</term><term>Catalysis</term><term>Horseradish Peroxidase (metabolism)</term><term>Hydrogen Peroxide</term><term>Polymers (chemical synthesis)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Catalyse</term><term>Dérivés du biphényle (métabolisme)</term><term>Horseradish peroxidase (métabolisme)</term><term>Peroxyde d'hydrogène</term><term>Polymères (synthèse chimique)</term><term>Spectrométrie de masse MALDI ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Polymers</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Biphenyl Compounds</term><term>Horseradish Peroxidase</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Dérivés du biphényle</term><term>Horseradish peroxidase</term></keywords><keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Polymères</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Catalysis</term><term>Hydrogen Peroxide</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Catalyse</term><term>Peroxyde d'hydrogène</term><term>Spectrométrie de masse MALDI</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry is a powerful tool for polymer characterization. It has been used to understand the enzymatic polymerization of 4-phenylphenol and to monitor number average molecular weight and weight average molecular weight of the polymer as a function of systematic addition of hydrogen peroxide (H(2)O(2)) in the reaction. A novel method, an introduction of internal standard for quantification of data, has been developed for MALDI-TOF MS to investigate the fate of each mers during the reaction. The preliminary data suggest that this approach provides new insight on the enzymatic synthesis, which is not available by other techniques. For the first time, we are able to understand the fate of several mers as a function of reaction conditions. The relative content of each mer increases with the addition of H(2)O(2), except for dimer and trimer. For example, the concentration of dimer species decreases as a function of H(2)O(2). On the other hand, the concentration of trimer species increases first and then decreases in the course of the reaction.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><noCountry><name sortKey="Cholli, Ashok L" sort="Cholli, Ashok L" uniqKey="Cholli A" first="Ashok L" last="Cholli">Ashok L. Cholli</name><name sortKey="Kumar, Jayant" sort="Kumar, Jayant" uniqKey="Kumar J" first="Jayant" last="Kumar">Jayant Kumar</name><name sortKey="Samuelson, Lynne" sort="Samuelson, Lynne" uniqKey="Samuelson L" first="Lynne" last="Samuelson">Lynne Samuelson</name></noCountry><country name="États-Unis"><region name="Massachusetts"><name sortKey="Xu, Peng" sort="Xu, Peng" uniqKey="Xu P" first="Peng" last="Xu">Peng Xu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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